Summary of the publications |
2023-2024 |
Hung The Nguyen, Takayuki Chikata, Yu Zhang, Giang Van Tran, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi, Role of HLA-B*58:01-restricted CD8+ T cells in HIV-1 subtype AE infection. J Infect Dis. In press |
HLA-B*58:01 and HLA-B*57 are protective alleles against HIV-1 subtype B or C infection whereas these HLA alleles have not been reported as protective in HIV-1 subtype AE infection. Although HLA-B*58:01-restricted and HLA-B*57-restricted HIV-1-specific CD8+ T cells have been thoroughly analyzed in subtype B or C infection, they have only been partially analyzed in subtype AE infection. We identified six HLA-B*58:01-restricted subtype AE epitopes in Vietnamese individuals infected with subtype AE. HLA-B*58:01-restricted T-cell responses to Gag epitopes, which may control disease progression in HLA-B*58:01+ and HLA-B*57+ individuals infected with subtype B or C, were not protective in subtype AE infection. These findings suggest that the loss of HLA-B*58:01-restricted T cells specific for some Gag epitopes and/or their reduced ability may account for the lack of protective effects conferred by HLA-B*58:01 in subtype AE infection. |
Nozomi Kuse, Osamu Noyori, Naofumi Takahashi, Yu Zhang, Shinya Suzu, and Masafumi Takiguchi, Recognition of HIV-1-infected fibrocytes lacking Nef-mediated HLA-B downregulation by HIV-1-specific T cells. J Virol. 98: e00791-24, 2024 |
Fibrocytes were reported
to be host cells for HIV-1, but the immunological recognition of HIV-1-infected
fibrocytes has not been studied. Here, we investigated the recognition of
HIV-1-infected fibrocytes by HIV-1-specific CD8+ T cells. CD8+ T cells specific
for five HIV-1 epitopes (HLA-A*24:02-restricted, HLA-B*52:01-restricted,
and HLA-C*12:02-restricted epitopes) produced IFN-ƒÁ and expressed CD107a
after coculture with HIV-1-infected fibrocytes. HIV-1-infected fibrocytes
were effectively killed by HIV-1-specific CD8+ T cells. Although it is well
known that HIV-1 Nef-mediated downregulation of HLA-A and HLA-B critically
affects the T cell recognition of HIV-1-infected CD4+ T cells and HIV-1-infected
macrophages, Nef downregulated HLA-A, but not HLA-B, in HIV-1-infected fibrocytes.
These findings suggested that HIV-1-specific CD8+ T cells could recognize
HIV-1-infected fibrocytes more strongly than HIV-1-infected CD4+ T cells
or HIV-1-infected macrophages. HIV-1-infected fibrocytes were also recognized
by HIV-1-specific HLA-DR-restricted T cells, indicating that HIV-1-infected
fibrocytes can present HIV-1 epitopes to helper T cells. Collectively, these
findings suggest that fibrocytes have an important role as antigen-presenting
cells during HIV-1 infection. The present study demonstrates effective recognition
of HIV-1-infected fibrocytes by HIV-1-specific T cells and suggests possible
roles of fibrocytes in the induction and maintenance of HIV-1-specific T
cells.
Importance: Fibrocytes were identified as unique hematopoietic cells with the features of both macrophages and fibroblasts and were demonstrated to be host cells for HIV-1. However, T cell recognition of HIV-1-infected fibrocytes has not been studied. We investigated the recognition of HIV-1-infected fibrocytes by HIV-1-specific T cells. HIV-1-infected fibrocytes were effectively recognized and killed by CD8+ T cells specific for HIV-1 epitopes presented by HLA-A, HLA-B, or HLA-C and were recognized by HIV-1-specific HLA-DR-restricted CD4+ T cells. HIV-1 Nef-mediated downregulation of HLA-A and HLA-B was found in HIV-1-infected CD4+ T cells, whereas Nef did not downregulate HLA-B in HIV-1-infected fibrocytes. These results suggest that HIV-1-specific CD8+ T cells recognize HIV-1-infected fibrocytes more strongly than HIV-1-infected CD4+ T cells. The present study suggests the importance of fibrocytes in the induction and maintenance of HIV-1-specific T cells. |
Nozomi Kuse, Hiroyuki Gatanaga, Yu Zhang, Takayuki Chikata, Shinichi Oka, and Masafumi Takiguchi, Epitope-dependent effect of long-term cART on maintenance and recovery of HIV-1-specific CD8+ T cells. J. Virol. 97: e01024-23, 2023 |
HIV-1-specific CD8+ T cells are anticipated to become effector cells for curative treatment using the "shock and kill" approach in people living with HIV-1 (PLWH) under combined antiretroviral therapy (cART). Previous studies demonstrated that the frequency of HIV-1-specific CD8+ T cells is reduced under cART and their functional ability remains impaired. These studies analyzed T-cell responses to a small number of HIV-1 epitopes or overlapping HIV-1 peptides. Therefore, the features of CD8+ T cells specific for HIV-1 epitopes under cART remain only partially clarified. Here, we analyzed CD8+ T cells specific for 63 well-characterized epitopes in 90 PLWH. We demonstrated that CD8+ T cells specific for large numbers of HIV-1 epitopes were maintained in an epitope-dependent fashion under long-term cART and that long-term cART enhanced or restored the ability of HIV-1-specific T cells to proliferate in vitro. This study implies that some HIV-1-specific T cells would be useful as effector cells for curative treatment. |
Takayuki Chikata, Hiroyuki Gatanaga, Hung The Nguyen, Daisuke Mizushima, Yu Zhang, Nozomi Kuse, Shinichi Oka, and Masafumi Takiguchi, HIV-1 protective epitope-specific CD8+ T cells in HIV-1-exposed seronegative individuals. iScience 26: 108089, 2023 |
Although previous studies have reported HIV-1-specific T cell responses in HIV-1-exposed seronegative (HESN) individuals, there has been no detailed analysis of these T cells against HIV-1 infection. We investigated HIV-1-specific CD8+ T cell responses in 200 Japanese HESN men who have sex with men (MSM). T cell responses to 143 well-characterized HIV-1 epitope peptides were analyzed by intracellular cytokine staining assay consisting of 3-week cultures of PBMCs stimulated with peptides. HLA-B*51:01-restricted Pol TI8-specific and HLA-A?02:06-restricted Pol SV9-specific CD8+ T cells were identified in two and one individuals, respectively, whereas CD8+ T cells specific for other HLA-A*02:06-restricted or HLA-B*51:01 epitopes were not present in these individuals. These epitope-specific T cells recognized HIV-1-infected cells. Because these two epitopes were previously reported to be protective in HIV-1-infected individuals, these protective epitope-specific T cells might suppress HIV-1 replication in HESN-MSM individuals. The present study suggests the contribution of protective epitope-specific T cells to protection against HIV-1 infection. |